smooth muscle protein 22 α sm22α  (Novus Biologicals)

Journal: Biotechnology Reports

Article Title: Xenogeneic-free platform for the isolation and scalable expansion of human bladder smooth muscle cells

doi: 10.1016/j.btre.2025.e00878

Figure Lengend Snippet: Isolation and characterization of bladder smooth muscle cells under xeno(geneic)-free culture conditions. A) Samples were fragmented into explants of approximately 1 mm diameter, and 10 explants per well were plated onto 6-well plates (Corning®). Phase contrast imaging of cells migrating and proliferating from explants (first image: donor 63 yo at day 2 post-processing; second image: donor 58 at day 6 post-processing; third and fourth images: donor 70 yo at day 6 post-processing); Scale bar: 100 µm. B) Representative images of fluorescence microscopy for the detection of typical SMC markers (red); left panel: donor 58 yo, cell passage 2; center panel: donor 80 yo, cell passage 5; right panel: donor 70 yo, Passage 3; Nuclei counterstained with DAPI (blue); Scale bar: 100 µm. C) Flow cytometry panel for SMC identity markers (positive and negative) for all four donors. αSMA: α-Smooth muscle actin; MYH11: Myosin heavy chain 11; SM22: Smooth Muscle Protein 22-α; SMC: Smooth muscle cells.

Article Snippet: Primary antibodies anti-human α-Smooth muscle actin (αSMA) (Invitrogen), Caldesmon, Desmin (Santa Cruz Biotechnology), Smooth Muscle Protein 22-α (SM22α) (Novus Biological), Myosin heavy chain 11 or smooth muscle myosin heavy chain (MYH11) (Abcam), CD44, CD90 and CD29 (Becton Dickinson, BD), were diluted in 1% (v/v) BSA and incubated for 2 h at RT.

Techniques: Isolation, Imaging, Fluorescence, Microscopy, Flow Cytometry

Journal: Biotechnology Reports

Article Title: Xenogeneic-free platform for the isolation and scalable expansion of human bladder smooth muscle cells

doi: 10.1016/j.btre.2025.e00878

Figure Lengend Snippet: Assessment of smooth muscle cell identity post-spinner flask culture. A) Live/dead staining assessed by flow cytometry immediately upon cell retrieval at the end of the spinner flask cultures for each donor. B) Flow cytometry for the depicted cell identity markers (positive and negative), C) Representative immunofluorescence images for SMC markers (red) and nuclei counterstained with DAPI (blue) on cells retrieved from microcarriers and adhered to static cultures; Upper panel: donor 58 yo; Middle panel: donor 80 yo; Bottom panel: donor 58 yo (for CD29 and CD44) and donor 80 yo (for CD90); Scale bar: 100 μm. αSMA: α-Smooth muscle actin; MYH11: Myosin heavy chain 11; SM22: Smooth Muscle Protein 22-α, SMC: smooth muscle cells.

Article Snippet: Primary antibodies anti-human α-Smooth muscle actin (αSMA) (Invitrogen), Caldesmon, Desmin (Santa Cruz Biotechnology), Smooth Muscle Protein 22-α (SM22α) (Novus Biological), Myosin heavy chain 11 or smooth muscle myosin heavy chain (MYH11) (Abcam), CD44, CD90 and CD29 (Becton Dickinson, BD), were diluted in 1% (v/v) BSA and incubated for 2 h at RT.

Techniques: Staining, Flow Cytometry, Cell Retrieval, Immunofluorescence

Journal: Advanced Materials Interfaces

Article Title: Preparation of ROS‐Responsive Exosome Coating of Nitinol Material for Making the Neurointerventional Stent

doi: 10.1002/admi.202300028

Figure Lengend Snippet: Figure 6. A) Expression of contractile VSMC marker proteins α-SMA and SM22α on the surface of BN and SMEN samples (scale bar = 50 µm). B) Fluorescence quantification of marker proteins α-SMA and SM22α. C) Transwell VSMCs–HUVECs co-culture effects of two groups of samples on VSMC migration (scale bar = 2 mm). *P ≤ 0.05 and **P ≤ 0.01.

Article Snippet: The cell polarization of VSMCs was induced with a serum-free medium containing platelet-derived growth factor BB subunits (HEGFP-16011; Oricell, Guangzhou, China) at 10 ng mL−1 for 24 h.[21] Then, the cell samples were washed, fixed, and incubated overnight with the primary antibody Smooth Muscle Protein 22-α (SM22α) (10493-1-AP, Proteintech, RRID: AB 2 199 363) or alpha smooth muscle actin (α-SMA) (14395-1-AP, Proteintech, RRID: AB 2 223 009).

Techniques: Expressing, Marker, Fluorescence, Co-Culture Assay, Migration